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Five Northern Virginia school districts are at risk of losing their federal funding after declining to comply with the Trump administration’s request to rescind policies supportive of transgender students, the Education Department announced on Tuesday.

The department’s Office for Civil Rights opened probes into the Arlington, Alexandria City, Fairfax, Loudoun and Prince William County school districts in February following requests to do so from America First Legal, the conservative nonprofit founded by White House deputy chief of staff Stephen Miller.

In a February letter to Craig Trainor, the Education Department’s acting assistant secretary for civil rights, America First Legal senior advisor Ian D. Prior alleged the districts were enforcing policies meant to support transgender students in violation of Title IX, the federal civil rights law against sex discrimination in schools.

The policies vary by district, but each allows transgender students to use restrooms and locker........

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